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Methods and Techniques

Our Biophysical Tools

Our research group investigates mechanics of viral replication, virus-host interactions, virus assembly and disassembly within the host. Specifically, we investigate energetics, structure and mobility of the intra-capsid genome as well as mechanical properties of viral capsids, influenced by changes in physiological environment that are important for viral replication. Main tools in our laboratory are: Atomic Force Microscopy (AFM), microcalorimetry, high resolution cryo electron microscopy, fluorescence microscopy (single-molecule FM and Super-Resolution FM), small angle x-ray and neutron scattering (SANS and SAXS).

 

Schematic representation of a virus capsid surface using atomic force microscopy. Graphical representation of the scanning principle of AFM.

Atomic Force Microscopy of viral genomes and capsids. 

 

 

Coloured Cryo-EM image showing a cross section of the bacteriophage lambda capsid with the DNA inside.

Cryo - Electron Microscopy of virus structure. 

 

 

Graphical representation of the isothermal titration calorimetry sample setup and representative figure of a measurement.

Microcalorimetry/energetics of viral genome release. 

 

 

Fluorescent confocal microscopy image of a cell nucleus in blue and attached viral capsids in green.

Super Resolution and Fluorescence Microscopy/ mechanism of viral infection and dynamics of viral populations. 

 

 

Example of an X-ray/neutron scattering curve and an image of the MAX IV facilities in Lund.

Small-angle X-ray and Neutron Scattering/structure of viral genomes.